Journal: Diagnostic Pathology

Article Title: Cytoplasmic FOXP1 expression is correlated with ER and calpain II expression and predicts a poor outcome in breast cancer

doi: 10.1186/s13000-018-0715-y

Figure Lengend Snippet: The correlation between cytoplasmic FOXP1 expression and clinicopathological parameters in IDC cases

Article Snippet: Following deparaffinization and heat-mediated antigen retrieval, immunohistochemical staining was carried out using an Envision system (DAKO, Glostrup, Denmark) with primary antibodies against FOXP1 (JC12, AbD Serotec, Oxford, UK), ER (SP1, Roche Tucson, AZ, USA), calpain II (CAPN2, Sigma, St. Louis, MO, USA), HER2 (4B5, Roche Tucson), pAKT (736E11, Cell signaling, Danvers, MA, USA), pmTOR (49F9, Cell signaling), p4E-BP1 (53H11, Cell signaling) and p-p70S6K (49D7, Cell signaling).

Techniques: Expressing

Journal: Diagnostic Pathology

Article Title: Cytoplasmic FOXP1 expression is correlated with ER and calpain II expression and predicts a poor outcome in breast cancer

doi: 10.1186/s13000-018-0715-y

Figure Lengend Snippet: The correlation between calpain II expression and clinicopathological parameters in IDC cases

Article Snippet: Following deparaffinization and heat-mediated antigen retrieval, immunohistochemical staining was carried out using an Envision system (DAKO, Glostrup, Denmark) with primary antibodies against FOXP1 (JC12, AbD Serotec, Oxford, UK), ER (SP1, Roche Tucson, AZ, USA), calpain II (CAPN2, Sigma, St. Louis, MO, USA), HER2 (4B5, Roche Tucson), pAKT (736E11, Cell signaling, Danvers, MA, USA), pmTOR (49F9, Cell signaling), p4E-BP1 (53H11, Cell signaling) and p-p70S6K (49D7, Cell signaling).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Protective Macroautophagy Is Involved in Vitamin E Succinate Effects on Human Gastric Carcinoma Cell Line SGC-7901 by Inhibiting mTOR Axis Phosphorylation

doi: 10.1371/journal.pone.0132829

Figure Lengend Snippet: SGC-7901 cells were treated with 0, 5, 10, 15, and 20 μg/mL VES for 24 h, and activation of p38 MAPK (p-p38 MAPK), Akt (p-Akt), AMPK (p-AMPK), mTOR (p-mTOR), p70S6K (p- p70S6K), and 4E-BP-1 (p-4E-BP-1) were examined using Western blot. The data in (A) and (C) were representative of three individual experiments with similar results. The data in (B) and (D) were expressed as mean ± S.D from three individual experiments. Actin was used as a loading control. * means p < 0.05 compared with control.

Article Snippet: Primary antibodies used for western blotting were rabbit anti-LC3 (Sigma, L7543), rabbit anti-Akt (Cell Signaling, 9272), rabbit anti-p-Akt (S473) (Cell Signaling, 9271S), rabbit anti-mTOR (Cell Signaling, 2972S), rabbit anti-p-mTOR (Ser2448) (Cell Signaling,5536S), rabbit anti-p70S6K (Cell Signaling,5707S), rabbit anti-p-p70S6K (T389) (Cell Signaling, 9205S), rabbit anti-4E-BP-1(Cell Signaling, 8594S), rabbit anti-p-4E-BP-1 (Thr37/46) (Cell Signaling, 9459S), rabbit antip38 MAPK (Cell Signaling,,8690S), rabbit anti-p-p38 MAPK (The180/Tyr1820) (Cell Signaling, 4092), rabbit anti-5'Adenosine monophosphate-activated protein kinase α (AMPKα) (Cell Signaling, 5831S), rabbit anti-p-AMPKα (Thr172) (Cell Signaling, 2535S) and rabbit anti-Actin (Santa Cruz Biotechnology, sc-1616).

Techniques: Activation Assay, Western Blot

Journal: PLoS ONE

Article Title: Protective Macroautophagy Is Involved in Vitamin E Succinate Effects on Human Gastric Carcinoma Cell Line SGC-7901 by Inhibiting mTOR Axis Phosphorylation

doi: 10.1371/journal.pone.0132829

Figure Lengend Snippet: VES induced the suppression of both Akt and p38 MAPK, and up-regulated AMPK, leading to the inhibition of mTOR activity. And downstream regulators p70S6K and 4E-BP-1 were suppressed consequently, resulting in autophagy in SGC-7901 cells. VES cooperated with autophagy inhibitors to induce increased anticancer effect compared with VES alone.

Article Snippet: Primary antibodies used for western blotting were rabbit anti-LC3 (Sigma, L7543), rabbit anti-Akt (Cell Signaling, 9272), rabbit anti-p-Akt (S473) (Cell Signaling, 9271S), rabbit anti-mTOR (Cell Signaling, 2972S), rabbit anti-p-mTOR (Ser2448) (Cell Signaling,5536S), rabbit anti-p70S6K (Cell Signaling,5707S), rabbit anti-p-p70S6K (T389) (Cell Signaling, 9205S), rabbit anti-4E-BP-1(Cell Signaling, 8594S), rabbit anti-p-4E-BP-1 (Thr37/46) (Cell Signaling, 9459S), rabbit antip38 MAPK (Cell Signaling,,8690S), rabbit anti-p-p38 MAPK (The180/Tyr1820) (Cell Signaling, 4092), rabbit anti-5'Adenosine monophosphate-activated protein kinase α (AMPKα) (Cell Signaling, 5831S), rabbit anti-p-AMPKα (Thr172) (Cell Signaling, 2535S) and rabbit anti-Actin (Santa Cruz Biotechnology, sc-1616).

Techniques: Inhibition, Activity Assay